MadSci Network: Molecular Biology

Subject: Is it possible to use a luciferase gene in a biology class experiment?

Date: Fri Apr 15 20:33:46 2005
Posted by David
Grade level: 10-12 School: Liberty Charter High
City: Nampa State/Province: ID Country: USA
Area of science: Molecular Biology
ID: 1113615226.Mb

Recently I've been researching bioluminescence, and found out that the gene
(luciferase) for the enzyme that fireflies use to glow is often used as an
indicator of successful implantation of a gene. I was wondering if it would be
possible to use it in the "genetic engineering" project that is done in my high
school's College Biology class. It usually involves trying to give some bacteria
resistance to a mild antibiotic via adding a gene and demonstrating that it
worked by introducing the antibiotic into the engineered and control cultures.
In the case of using luciferase, I just thought it would be neat to make some
glowing bacteria - it's a much more 'visual' effect for showing results. Is
there a company that would sell the gene to a high school teacher? Would there
be any legal problems with this? Other kinds of problems?

Re: Is it possible to use a luciferase gene in a biology class experiment?

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