MadSci Network: Molecular Biology

Re: Is it possible to use a luciferase gene in a biology class experiment?

Date: Mon Apr 18 08:30:18 2005
Posted By: Allen Gathman, Faculty, Biology, Southeast MO St. U.
Area of science: Molecular Biology
ID: 1113615226.Mb

I don't know of a luciferase-based teaching kit. However, a similar dramatic effect can be achieved using green fluorescent protein (GFP), which comes from the fluorescent jellyfish Aequorea victoria.

Bio-Rad company ( sells a "pGLO bacterial transformation kit" that allows students to transform E. coli with a plasmid containing the GFP gene.

It does require a UV source in order to see the fluorescence. We've used it in classes at the university level with good success.

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