Subject: Frog Nuclei Transfer Procedure Question.

I wish to attempt a a nuclear transfer of a frog. I plan to take a frog egg and
remove the nucleus. I want to then insert it into a new egg. Theorically it
should form a female frog with no father. How can i isolate the nucleus and
insert it in the new cell. Should i use a micropipette, if so what size and
where could i find it. Another problem would be how to activate the new egg. I
suppose a simple heat shock or an injection of Ca+ ions, but i do not know if
this would help? Please Help!

Re: Frog Nuclei Transfer Procedure Question.