|MadSci Network: Development|
I wish to attempt a a nuclear transfer of a frog. I plan to take a frog egg and remove the nucleus. I want to then insert it into a new egg. Theorically it should form a female frog with no father. How can i isolate the nucleus and insert it in the new cell. Should i use a micropipette, if so what size and where could i find it. Another problem would be how to activate the new egg. I suppose a simple heat shock or an injection of Ca+ ions, but i do not know if this would help? Please Help!
Re: Frog Nuclei Transfer Procedure Question.
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