MadSci Network: Development

Re: Frog Nuclei Transfer Procedure Question.

Date: Tue Dec 19 13:33:06 2006
Posted By: Paul Szauter, Staff, Mouse Genome Informatics
Area of science: Development
ID: 1164848208.Dv

This experiment is a great deal harder to accomplish technically than it first seems from the 
description. You would almost certainly need access to expensive instruments (an excellent 
microscope and a device for "pulling" micropipettes from capillary tubes). You are also not likely 
to succeed using a written protocol without expert technical assistance right at your side.

Here is an excellent classic reference on this experiment:

There is also a great answer in the MadSci archive:


Paul Szauter
Mouse Genome Informatics

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