MadSci Network: Physics
Query:

Re: Connection between wavelength 405, 450, 492nm and absorbances 2,4;1,417;0,8

Date: Thu Mar 1 02:34:33 2007
Posted By: Zehra Sarac, PhD, Department of Electric and Electronic Engineering , Zonguldak Karaelmas University
Area of science: Physics
ID: 1172578041.Ph
Message:

Dear Lilijana

In spectroscopy, the absorbance A is defined as
 
A(wavelength)=log10(Io/I)

where I is the intensity of light at a specified wavelength ë that has 
passed through a sample (transmitted light intensity) and I0 is the 
intensity of the light before it enters the sample or incident light 
intensity. Absorbance for same object can be different in different 
wavelengths.. So you can find a wavelength,that the absorbance is maximum 
in this wavelength...

Best wishes




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