MadSci Network: Molecular Biology
Query:

Subject: how do you design primers for PCR if you don't know what the DNA sequence?

Date: Sat Mar 1 17:59:31 2008
Posted by No name entered.
Grade level: 10-12 School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Molecular Biology
ID: 1204419571.Mb
Message:

Primers have to be made so that DNA polymerase can elongate a DNA template. but
what happens if you don't know the sequence? 


Re: how do you design primers for PCR if you don't know what the DNA sequence?

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