|MadSci Network: Molecular Biology|
Hello, It's quite simple : if you do not have the actual sequence of the DNA you want to amplify, or at least the 3' and 5' ends, then you cannot design primers and run conventional PCR. But there are many ways to amplify DNA. Search your literature for the following keywords : SISPA, phi29 polymerase, multiple displacement, RDA, and isothermal amplification. I advise you the papers from Allander et al., which are quite detailed and give a good explanation... Enjoy
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