MadSci Network: Biochemistry |
Would anyone be kind enough to explain to me what is titration...I am trying to design a lab regarding the rate of enzyme activity. I am using different concentrations of substrate and it is suppose to be like a logarithmic function (increase dramatically in rate of reaction and gradually levels off as concentration continues to increase) So I will be using hydrogen peroxide H2O2 as substrate and cow liver as the enzyme... After the reactions, I will be titrating (see link http://bioweb.wku.edu/courses/Biol114/enzyme/enzyme2.asp) and according to this website, the amount of titrate used (KMnO4) is directly proportional to the amount of substrate left...? I am not sure if this will help me in the rate of enzyme activity... Thanks
Re: What is Titration and how does it work???
Try the links in the MadSci Library for more information on Biochemistry.