MadSci Network: Biochemistry
Query:

Subject: What is Titration and how does it work???

Date: Sat Feb 23 08:17:27 2008
Posted by No name entered.
Grade level: 10-12 School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Biochemistry
ID: 1203779847.Bc
Message:

Would anyone be kind enough to explain to me what is titration...I am trying 
to design a lab regarding the rate of enzyme activity.

I am using different concentrations of substrate and it is suppose to be like 
a logarithmic function (increase dramatically in rate of reaction and 
gradually levels off as concentration continues to increase)

So I will be using hydrogen peroxide H2O2 as substrate and cow liver as the 
enzyme...

After the reactions, I will be titrating (see link 
http://bioweb.wku.edu/courses/Biol114/enzyme/enzyme2.asp) and according to 
this website, the amount of titrate used (KMnO4) is directly proportional to 
the amount of substrate left...?

I am not sure if this will help me in the rate of enzyme activity...

Thanks


Re: What is Titration and how does it work???

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