Re: What is Titration and how does it work???

Titration provides a way to measure the amount of some chemical or reagent. You add a different reagent that reacts with the chemical of interest. You add that test reagent in small aliquots (volumes) until some indicator changes -- color, pH if you were using a pH meter.

What makes the titration a "measurement" is that you are keeping track of how much test reagent you add. This enable the amount of test reagent added to be correlated to the condition under which you make the test. In your virtual lab exercise, you are determining the amount of residual hydrogen peroxide by colorimetric change. Each tube contains the same amount of enzyme, and you are measuring the enzyme activity by its ability to convert hydrogen peroxide to water and oxygen. You do this indirectly by measuring the amount of peroxide that remains after the catalase reaction proceeds for a given time. Less catalase activity -- more residual peroxide and more permanganate needs to be added.

So you can relate catalase activity to the amount of permanganate needed to be added to get the colorimetric change. That is the titration, and the titration quantitatively relates the amount of permanganate added to the activity of the enzyme. The enzyme activity is determined by (i) the amount of enzyme, and (ii) its thermodynamics, which are determined by the environmental conditions, temperature in this case. So you can relate catalase activity to reaction temperature by titrating the amount of residual peroxide. The residual peroxide is titrated by adding permanganate.