MadSci Network: Chemistry

Subject: Citrate phosphate buffer preparation

Date: Tue Mar 3 04:48:51 2009
Posted by No name entered.
Grade level: grad (science) School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Chemistry
ID: 1236080931.Ch

How do i prepare a citrate phosphate buffer(0.1M,pH 6.0)and 0.05M,pH 6.0)? From
literature search, i should  use citric acid and sodium phosphate. But i am
getting lost the more i read.I'd wish to use it as an extraction buffer for an
enzyme assay. Thanks

Re: Citrate phosphate buffer preparation

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