MadSci Network: Chemistry

Re: Citrate phosphate buffer preparation

Date: Tue Apr 14 01:56:26 2009
Posted By: Sebastien De Landtsheer, Undergraduate, Immunology, Laboratoire National de Santé
Area of science: Chemistry
ID: 1236080931.Ch


I might not have grasped all the subtilities, but it looks quite obvious 
to me : Per liter, add 0.1mol citric acid (19.212g) and 0.05mol sodium 
biphosphate (6.9g). Then raise the pH to 6.0 with 1M NaOH.
You may also calculate, in your final solution, the concentrations of the 
different ions, and then add them individually (Na citrate + citric acid 
+ phosphoric acid + monophosphate + diphosphate) but that's not how it is 
usually made in a lab, equiped with a pH-meter.

Hope that helps

Current Queue | Current Queue for Chemistry | Chemistry archives

Try the links in the MadSci Library for more information on Chemistry.

MadSci Home | Information | Search | Random Knowledge Generator | MadSci Archives | Mad Library | MAD Labs | MAD FAQs | Ask a ? | Join Us! | Help Support MadSci

MadSci Network,
© 1995-2006. All rights reserved.