Re: Citrate phosphate buffer preparation

Hello, 

I might not have grasped all the subtilities, but it looks quite obvious 
to me : Per liter, add 0.1mol citric acid (19.212g) and 0.05mol sodium 
biphosphate (6.9g). Then raise the pH to 6.0 with 1M NaOH.
You may also calculate, in your final solution, the concentrations of the 
different ions, and then add them individually (Na citrate + citric acid 
+ phosphoric acid + monophosphate + diphosphate) but that's not how it is 
usually made in a lab, equiped with a pH-meter.

Hope that helps