|MadSci Network: Biochemistry|
When I read this protocol (http://www.natureprotocols.com/2007/08/02/detecting_antigens_by_quantita.php) and other ones as well, I found that in the washing step before blocking,they used TBS. However, in all washing step after blocking, they used TETBS (tween and TBS). I have tried to explain this changing however I can't find a clear answer. It would be great if you could tell me why they did so. Thanks a lot!
Re: Washing in Immuno-PCR test. (second question)
Try the links in the MadSci Library for more information on Biochemistry.