MadSci Network: Biochemistry

Re: Washing in Immuno-PCR test. (second question)

Date: Thu Aug 19 14:35:17 2010
Posted By: Karin Crowhurst, Assistant Professor, Biochemistry
Area of science: Biochemistry
ID: 1278393072.Bc

Hi Huong,

I am not a pro when it comes to these types of methods, but hopefully this answer will help:

In general for these types of procedures, Tween helps to wash away unbound blocking agent (the BSA in the BTBS solution). In other ELISA-type procedures, or steps in this procedure, it can also prevent non-specific antibody binding (or washes away unbound antibody). Tween is a type of detergent, so its general use is to help keep moleculaes separated (prevent interactions, aggregations, etc.).

Hope this helps!

Current Queue | Current Queue for Biochemistry | Biochemistry archives

Try the links in the MadSci Library for more information on Biochemistry.

MadSci Home | Information | Search | Random Knowledge Generator | MadSci Archives | Mad Library | MAD Labs | MAD FAQs | Ask a ? | Join Us! | Help Support MadSci

MadSci Network,
© 1995-2006. All rights reserved.