| MadSci Network: Botany |
Ian,
The first thing you need to do is a little research in order to develop a
good hypothesis. Look for information on plant photosynthesis and light
wavelengths. You should have in mind what wavelengths plants use and what
wavelengths each of your treatments provides; then you will be able to make
a good hypothesis based on the information you find.
It sounds like you're on the right track with the experiment itself. You
will need to think about what kind of plant you want to use. I would look
for something that has a short life cycle (if you use garden seeds, the
packet will indicate this as "days to harvest") and that will be easy to
measure (not a vine, like pole beans or peas, or a rosette, like radish or
cabbage). A few controls that I would suggest are:
1. Make sure that all factors other than light are the same for all
your plants (try to keep the temperature the same, keep them all well
watered, etc.)
2. Keep the duration of light the same for the sunlight and black light
treatments; i.e., turn your black light on at sunrise and off at sunset.
That way you know that any differences you observe are due only to
wavelength and not to how long the plants received light.
You should also make sure that you have several replications within each
treatment. That is, grow 5 or 10 (or as many as you have room for) plants
in sunlight, and the same number in black light and in the dark. When you
are done with the experiment, you can average the results of all the plants
in each treatment and compare the averages. Just like us, plants have
genetic variability; some individual plants will grow better than others
just because their genes are different. If you only have one plant in each
treatment, you can't be sure that the differences you see aren't just
individual differences in the plants' genes. The more plants you use, the
more confidence you can have that your results aren't due to these
individual differences.
As you are setting up, think about what kinds of measurements you will be
taking, how often, and for how long. How are you going to determine "good"
versus "bad" responses? Survival for some period of time? Leaf size? Leaf
color? Number of leaves? Plant height? Development of flowers and fruit?
Again, a little research will help you determine how you should judge your
plants' growth. Some plant responses to adverse conditions will make
measurements like height unreliable by themselves (hint: look up
etiolation), so you should consider using a group of characteristics to
describe the condition of your plants.
Good luck, and have fun!
Brantlee Spakes
Department of Plant Biology
Arizona State University
Box 871601
Tempe, AZ 85287-1601
Try the links in the MadSci Library for more information on Botany.