MadSci Network: Molecular Biology
Query:

Subject: Why is DNA resistant to phenol denaturation, yet proteins aren't?

Date: Thu May 4 04:06:57 2000
Posted by lisa
Grade level: undergrad School: No school entered.
City: No city entered. State/Province: No state entered. Country: Australia
Area of science: Molecular Biology
ID: 957431217.Mb
Message:

This question refers to the isolation & purification of plasmid DNA 
molecular biology protocol. A combination of phenol,chloroform and isoamyl 
alcohol are used to extract DNA from contaminants, namely proteins and 
many types of RNA molecules. The steps of the DNA ladder are made up of 
nucleotide bases that code for amino acids and thus ultimately proteins. 
I'm not sure if DNA is protected from denaturation by phenol by the actual 
phosphoribose backbone or if there is some other reason. I'm also unable 
to find out, beyond the fact that phenol denatures proteins,how it 
actually does this.


Re: Why is DNA resistant to phenol denaturation, yet proteins aren't?

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