MadSci Network: Molecular Biology |
This question refers to the isolation & purification of plasmid DNA molecular biology protocol. A combination of phenol,chloroform and isoamyl alcohol are used to extract DNA from contaminants, namely proteins and many types of RNA molecules. The steps of the DNA ladder are made up of nucleotide bases that code for amino acids and thus ultimately proteins. I'm not sure if DNA is protected from denaturation by phenol by the actual phosphoribose backbone or if there is some other reason. I'm also unable to find out, beyond the fact that phenol denatures proteins,how it actually does this.
Re: Why is DNA resistant to phenol denaturation, yet proteins aren't?
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