| MadSci Network: Microbiology |
The way that heat is applied to water has very little to do with
whether bacteria in the water live or die. What matters is the
temperature. Most of the things in water that might make us sick
(bacteria, viruses, parasites) are killed by boiling, but some bacteria
form spores that are not killed by boiling. Fortunately, they are not
common in water and are not likely to cause disease if water containing
them is drunk.
The key to your question is that water (pure, at sea level) boils
at 212 degrees Fahrenheit (100 degrees Celsius), no matter how the heat is
applied. If you put a pan of water to boil on a stove top burner and
measure the temperature with the right kind of thermometer, it will be
somewhere near 212 — depending on your altitude and the atmospheric
pressure that day. If you put another pan of water in the oven at, say,
350 degrees Fahrenheit, the water won't get any hotter than it did on the
stove top because it keeps itself "cool" by boiling. A thermometer will
prove this. When all of the water has boiled away, the pan will get to
350. If we want to kill the bacteria that form spores, we do what the
canning companies do — use a pressure cooker to raise the boiling point
(temperature) of the water.
So, you can show that the temperature of boiling water is the same
as the temperature of "baking" water with a thermometer that will register
such temperatures (say, a candy thermometer). If you dissolve a lot of
salt or sugar in the water, you can raise its boiling temperature
significantly.
If you want to know whether the bacteria have all died, you can buy
some sterile "petri plates" of medium (say, nutrient agar, for a common
one) and spread a few drops on the agar surface. You can transfer the
water, a drop at a time, from the pan to the agar with a metal or glass
rod that has been wiped off with rubbing alcohol and let dry. You'll need
to put a few drops of unheated water on one plate, a few drops of boiled
water on another, and a few drops of "baked" water on a third. "Disinfect"
your rod again with rubbing alcohol after you finish each plate. Technique
is tricky, though; it's very easy to get bacteria from fingers, your
breath, or the air onto the agar surface and ruin the experiment. You
can touch the agar in another plate with just the "disinfected" rod (no
water) to see whether anything dropped in that was not from the water. Put
the lid back on each plate as soon as you have put maybe 4 drops of water
at various places on the agar surface. After a half hour, carefully turn
the plates over and put them somewhere where the temperature is comfortable
(for you) and preferably away from light. Look at them the next morning,
and daily till the agar dries out. You should see little clumps of
bacteria ("colonies") growing on the agar surface where the water drops
were. If there were a lot of bacteria, the whole area wet by the water
drops will be covered by bacterial growth. If boiling or baking the water
killed all the bacteria, you won't see anything where those drops landed.
You may see a few after a couple of days, if there were spores in the
water.
You can try to look for water bacteria with a microscope, but
that's not a really good way to do this kind of experiment because dead
bacteria (killed by the heat) may stay around for some time and look just
like the ones that were alive. Preparing bacteria to be seen with a
microscope requires "fixing" them (mild heating kills them and sticks them
to a glass slide). It is usually necessary to stain the bacteria and to
look at them with a microscope lens that needs oil under it to magnify the
bacteria enough to be seen. If you really want to see the bacteria, rather
than just learn whether they are alive or dead, you will need to find a
nearby science teacher to give you a hands-on lesson.
Good luck!
Try the links in the MadSci Library for more information on Microbiology.