| MadSci Network: Botany |
If you can, it would be best to use two beakers, one inside the other. Put the Elodea in the smaller beaker and place it inside a large beaker that has the food coloring solution in it. That way there is no possibility the food coloring is having a toxic effect on the Elodea, it will just be changing the light color the Elodea receives. You may need to place an opaque cover on the Elodea beaker to assure all the light the Elodea gets is passing through the food coloring solution. If your school has a spectrophotometer, it would be desirable to do a transmission graph for each food coloring solution. Measure the percent transmission every 5 or 10 nanometers between wavelengths of 400 and 700 nanometers (equivalent to 0.4 to 0.7 micrometers). Graph the percent transmission on the vertical axis versus wavelength on the horizontal axis. The transmission graphs are useful because the human eye is not a good light sensor. A green food coloring solution will transmit mainly green wavelengths, but it may be transmiting other colors as well. The human eye is most senstive to green wavelengths and much less sensitive to red and blue wavelengths. It is hard to predict your results because they will depend on the light transmission of the various food coloring solutions and the light source. For example, incandescent bulbs are very rich in red wavelengths compared to sunlight and fluorescent lamps. You may find slightly lower photosynthesis with green or yellow compared to red or blue but that is far from guaranteed. Contrary to many books, leaves do not reflect all green light. Check a photosynthesis action spectrum to see how light color or wavelength affects photosynthesis rate. To fairly compare the effect on photosynthesis of different light colors requires an expensive PAR meter to assure each color provides the same amount of light. References Re: Do different colors of light affect the growing rate/ability of plants? Re: what is meant by an action spectrum as opposed to an absobrtion spectrum Re: How much light does chlorophyl absorb? How much other materials? Examples of transmission spectrum graphs Solar Spectrum Graph Incandescent (Tungsten) Lamp Spectrum Graph Fluorescent Lamp Spectrum Graph
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