Connection between wavelength 405, 450, 492nm and absorbances 2,4;1,417;0,8

Subject: Connection between wavelength 405, 450, 492nm and absorbances 2,4;1,417;0,8

Date: Tue Feb 27 05:07:21 2007
Posted by Lilijana
Grade level: grad (science) School: Institute for public health
City: Nova Gorica State/Province: No state entered. Country: Slovenija
Area of science: Physics
ID: 1172578041.Ph

Message:


We have some problems with ELISA reader (spectrophotometer): we are not sure if 
filters of 405, 450 and 492 are on right positions.
We measured one sample (of yellow color) at supposed 405, 450 and 492 nm and 
found absorbances: 2,400 ; 1,417 ; 0,829. Is it possible to connect different 
absorbances of the same sample with different waveleghts? For example 
absorbance 2,400 was read at wavelength 450 nm?
With best wishes!
Lilijana Merljak

Re: Connection between wavelength 405, 450, 492nm and absorbances 2,4;1,417;0,8

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