Re: What are the best Micrococcus luteus growth conditions?

It should work on the agar you have selected but at 30C you may not see any
growth for 3-5 days.  Micrococcus luteus is not a fastidious organism to
grow it just has very small cells so colonies are not visible for a little
bit longer time.  When I was in the lab we would grow M. luteus on soybean
casein digest agar at 35-39C with a 48-72 hour incubation time.  When it
was grown at 30C we would wait 72-96 hour incubation.

How are you suspending your frozen culture? Are you taking a lyophilized
pellet?  If so, try placing the pellet into sterile saline 0.9% and
vortexing or mixing it really well.  Then swab it or spread plate it onto
your agar plate for a bacterial lawn.

If you are still not seeing growth after 3-5 days then there is a chance
that your frozen culture is no longer viable.  

Best of luck!