| MadSci Network: Biochemistry |
I am trying to measuring lipase activity(from fish) using substrate pNPP(p- nitrophenyl palmitate) which would form pNP upon lipase react on it. I am using spectrophotometer at 410nm to measure pNP formation. And I am not sure how do I calculate extinction coefficient of my substrate product pNP. Do I measure the Absorbance of pNP at different concentration, and graph the result. Is the slope of the graph my pNP extinction coefficient? What is the unit then? Thank you!
Re: How do I calculate extinction coefficient of my product pNP
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