Can proteinase K inactivate lysozymes?

Subject: Can proteinase K inactivate lysozymes?

Date: Wed Oct 6 04:17:48 1999
Posted by Daniel Ericsson
Grade level: undergrad School: Swedish agricultural university
City: Uppsala State/Province: Uppland Country: Sweden
Area of science: Biochemistry
ID: 939197868.Bc

Message:


In preparation of chromosomal DNA from E.Coli lysozymes are added to digest
the cellwalls further (they have already been broken up by deepfeezing and 
subsequent thawing).Proteinase K is then added and I wonder why; is it to 
abort the lysozyme reaction or perhaps to furhter prepare the DNA?

Re: Can proteinase K inactivate lysozymes?

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