MadSci Network: Chemistry |
I am asking this question as I am doing my chemistry A-level coursework. I am looking at what's in aspartame. This includes investigating the best conditions needed for the hydrolysis of the peptide bonds, by changing the concentration of acid and reflux time. Once the aspartame has been hydrolysed I have preformed paper chromatography to look at what is present in the solvent by comparing it with aspartic acid and phenylalanine. I would also like to find the concentration of solvent present. So I was thinking of preforming a titration but I did not know with what as I will try one thing and it will react with one of the other prodcuts. (methanol,aspartic acid or phenylalanine) I thought that the best product that would be able to give me a concentration would be methanol as it would be hard to find something that would not react with both the amino acids present? I would be very grateful if you could help me as soon as possible as both my teacher and I have been trying to think of a way to find the concentration for quite a while.( I am doing the Salters A-level course(MOD 5B,Syllabus 5688 OCR) and my experiment is based on the subject topic engineering proteins experiment EP2.2 )
Re: A test that can give the concentration of aspartame after hydrolysis?
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